Enzyme Assay for Identification of Pectin and Pectin Derivatives, Based on Recombinant Pectate Lyase
نویسندگان
چکیده
منابع مشابه
Enzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.
A simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from Aspergillus niger. When pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between C-4 and C-5 in the galacturonic acid residue of the released nonreducing end. The formation of double bonds produced an increase in light...
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The gene pelA encoding a pectate lyase from the strain Bacillus sp. BP-23 was cloned and expressed in Escherichia coli. The nucleotide sequence of a 1214 bp DNA fragment containing pelA gene was determined, revealing an ORF of 666 nucleotides that encoded a protein of 23233 Da. The deduced amino acid sequence of the encoded enzyme showed homology to pectate lyases A, B, C and D from Fusarium so...
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The gene yvpA from Bacillus subtilis was cloned and expressed in Escherichia coli. It encoded a pectate lyase of 221 amino acids that was denominated PelC. The heterologously expressed enzyme was purified by His-tag affinity chromatography and characterized. PelC depolymerized polygalacturonate and pectins of methyl esterification degree from 22 % to 89 %, exhibiting maximum activity on 22 % es...
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Villum Kann Rasmussen Centre Pro-active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, DK–1871 Frederiksberg C, Copenhagen, Denmark (J.H.); Feedstocks Division, Joint BioEnergy Institute, Emeryville, California 94608 (A.S., H.V.S.); and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, California 94608 (...
متن کاملDiscovery of pectin-degrading enzymes and directed evolution of a novel pectate lyase for processing cotton fabric.
There is a growing need in the textile industry for more economical and environmentally responsible approaches to improve the scouring process as part of the pretreatment of cotton fabric. Enzymatic methods using pectin-degrading enzymes are potentially valuable candidates in this effort because they could reduce the amount of toxic alkaline chemicals currently used. Using high throughput scree...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 2001
ISSN: 1060-3271,1944-7922
DOI: 10.1093/jaoac/84.6.1851